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| 4. |  | MARQUES, H. P.; SIQUEIRA, F. G. de; LEAO, A. P.; SOUZA JUNIOR, M. T. Otimização de métodos de extração de DNA genômico dos basidiomicetos Panus lecomtei CC40 e Pleurotus pulmonarius EF88. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 4.,2017, Brasília, DF. Anais... Brasília, DF: Embrapa, 2017. p. 33-38.| Biblioteca(s): Embrapa Agroenergia. |
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| 5. | | ROSADO, T. B.; LEAO, A. P.; SANTOS, R. F. dos; LAVIOLA, B. G.; GRATTAPAGLIA, D. Taxa de cruzamento em pinhão manso estimada por meio de marcadores RAPD. In: CONGRESSO DA REDE BRASILEIRA DE TECNOLOGIA DE BIODIESEL, 4.; CONGRESSO BRASILEIRO DE PLANTAS OLEAGINOSAS, ÓLEOS, GORDURAS E BIODIESEL, 7., 2010, Belo Horizonte. Biodiesel: inovação tecnológica e qualidade: anais. Lavras: UFLA, 2010. v. 1 p. 127-128.| Biblioteca(s): Embrapa Agroenergia. |
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| 6. | | ALVES, A. A.; PEREIRA, V. M.; LEAO, A. P.; FORMIGHIERI, E. F.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Advancing palm genomics by developing a high-density battery of molecular markers for Elaeis oleifera for future downstream applications. In: CONGRESS OF THE BRAZILIAN OF BIOTECHNOLOGY, 5., 2013, Florianópolis, SC. Abstracts... Brasília, DF: Sociedade Brasileira de Biotecnologia, 2013.| Biblioteca(s): Embrapa Agroenergia. |
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| 8. | | NASCIMENTO, M. S.; SOUZA, R. A. V.; CAMILLO, J.; LEAO, A. P.; LAVIOLA, B. G.; SOUZA JUNIOR, M. T. Enraizamento e aclimatização de plantas de pinhão-manso oriundas da organogenese a partir de discos cotiledonares. In: CONGRESSO BRASILEIRO DE PESQUISA EM PINHÃO-MANSO, 2., 2011, Brasília, DF. Pinhão-manso: focando em soluções sustentáveis para produção de biocombustíveis: anais. Brasília, DF: Embrapa Agroenergia: ABPPM, 2011. 1 CD-ROM. (Embrapa Agroenergia. Documentos, 005). Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 10. | | MORAIS, P. B.; CAPDEVILLE, G. de; FARIAS, M. P.; SOUZA JUNIOR, M. T.; FALCÃO, R.; LEAO, A. P. Estudo do desenvolvimento meiótico de cromossomos em microsporócitos coletados de inflorencências de Elaeis oleifera subamostra coari. In: ENCONTRO DE PESQUISA E INOVAÇÃO DA EMBRAPA AGROENERGIA, 2., 2015, Brasília, DF. Anais ... Brasília, DF: Embrapa Agroenergia, 2015. p. 118-120| Biblioteca(s): Embrapa Agroenergia. |
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| 11. | | NASCIMENTO, M. S.; SOUZA, R. A. V.; CAMILLO, J.; LEAO, A. P.; LAVIOLA, B. G.; SOUZA JUNIOR, M. T. Multiplicação de plantas de pinhão manso via organogênese a partir de discos cotiledonares. In: CONGRESSO BRASILEIRO DE PESQUISA EM PINHÃO-MANSO, 2., 2011, Brasília, DF. Pinhão-manso: focando em soluções sustentáveis para produção de biocombustíveis: anais. Brasília, DF: Embrapa Agroenergia: ABPPM, 2011. 1 CD-ROM. (Embrapa Agroenergia. Documentos, 005). Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 12. | | ALVES, A. A.; FORMIGHIERI, E. F.; CAMILLO. J.; LEAO, A. P.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Revisiting an Unfinished Business: the Genome Content of Elaeis guineensis, E. oleifera and its Inter-specific Hybrid. In: INTERNATIONAL PLANT AND ANIMAL GENOME, 20., 2012, San Diego, USA. Anais... Wuerselen: Lemna Tec GmbH, 2012. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 14. | | FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. Structural and functional analysis of stress-resonsive genes and their selected promoters from young oil palm (ELAEIS GUINEENSIS) under two abiotic stresses. In: BRAZILIAN CONGRESS OF GENETICS, 67., 2022, Natal, RN. Porto Alegre: Sociedade Brasileira de Genética, 2022. p. 320| Biblioteca(s): Embrapa Agroenergia. |
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| 16. | | SOUZA JUNIOR, M. T.; CAPDEVILLE, G. de; FORMIGHIERI, E. F.; CAMILLO, J.; ALVES, A. A.; LEAO, A. P. Whole Genome Sequencing of the E. oleifera South-American Wild Oil Palm. In: INTERNATIONAL PLANT AND ANIMAL GENOME, 20., 2012, San Diego, USA. Anais... Wuerselen: Lemna Tec GmbH, 2012.| Biblioteca(s): Embrapa Agroenergia. |
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| 17. | | VARGAS, L. H. G.; LEAO, A. P.; FARIAS. P. de F.; ALVES, A. A.; FORMIGHIERI, E. F.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Avaliação da fragmentação do DNA genômico de Elaeis guineensis Jacq e Elaeis oleífera (Kunth) Corté por meio de ondas sonoras de alta frequência e autoclavagem. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado.| Biblioteca(s): Embrapa Agroenergia. |
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| 18. | | FARIAS, M. P.; VARGAS, L. H. G.; LEAO, A. P.; FORMIGHIERI, E. F.; ALVES, A. A.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Determinação de estádios meióticos em elaeis oleifera (kunth) cortés. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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| 19. | | LEAO, A. P.; VARGAS, L. H. G.; FARIAS, M. P.; FORMIGHIERI, E. F.; ALVES, A. A.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Estabelecimento de curva de melting para elaeis guineenses jacq. e elaeis oleífera (kunth) cortés. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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| 20. | | VARGAS, L. H. G.; LEAO, A. P.; FARIAS, M. P.; ALVES, A. A.; FORMIGHIERI, E. F.; CAPDEVILLE, G. de; SOUZA JUNIOR, M. T. Estabelecimento de um protocolo eficiente para extração de rna a partir de folhas jovens de elaeis guineensis jacq. In: WORKSHOP AGROENERGIA, 6., 2012, Ribeirão Preto, SP. Anais... Ribeirão Preto: APTA; Campinas: IAC, 2012. Não paginado| Biblioteca(s): Embrapa Agroenergia. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agroenergia. Para informações adicionais entre em contato com cnpae.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Agroenergia; Embrapa Meio-Norte. |
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Data corrente: |
01/11/2022 |
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Data da última atualização: |
01/11/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, T. M. M.; FERREIRA FILHO, J. A.; LEAO, A. P.; SOUSA, C. A. F. de; SOUZA JUNIOR, M. T. |
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Afiliação: |
THALITA MASSARO MALHEIROS FERREIRA; JAIRE ALVES FERREIRA FILHO; ANDRE PEREIRA LEAO, CNPAE; CARLOS ANTONIO FERREIRA DE SOUSA, CPAMN; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
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Título: |
Structural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
BMC Genomics, v. 23, n. 735, p. 1-13, 2022. |
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DOI: |
https://doi.org/10.1186/s12864-022-08926-6 |
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Idioma: |
Inglês |
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Conteúdo: |
Background Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance. MenosBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, trans... Mostrar Tudo |
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Palavras-Chave: |
QRT-PCR; RNA-Seq. |
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Thesaurus NAL: |
Abiotic stress; Salinity; Transcriptomics. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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Marc: |
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001 2147944
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024 7 $ahttps://doi.org/10.1186/s12864-022-08926-6$2DOI
100 1 $aFERREIRA, T. M. M.
245 $aStructural and functional analysis of stressinducible genes and their promoters selected from young oil palm (Elaeis guineensis) under salt stress.$h[electronic resource]
260 $c2022
520 $aBackground Soil salinity is a problem in more than 100 countries across all continents. It is one of the abiotic stress that threatens agriculture the most, negatively affecting crops and reducing productivity. Transcriptomics is a technology applied to characterize the transcriptome in a cell, tissue, or organism at a given time via RNA-Seq, also known as full-transcriptome shotgun sequencing. This technology allows the identification of most genes expressed at a particular stage, and different isoforms are separated and transcript expression levels measured. Once determined by this technology, the expression profile of a gene must undergo validation by another, such as quantitative realtime PCR (qRT-PCR). This study aimed to select, annotate, and validate stress-inducible genes?and their promoters?differentially expressed in the leaves of oil palm (Elaeis guineensis) plants under saline stress. Results The transcriptome analysis led to the selection of 14 genes that underwent structural and functional annotation, besides having their expression validated using the qRT-PCR technique. When compared, the RNA-Seq and qRT-PCR profiles of those genes resulted in some inconsistencies. The structural and functional annotation analysis of proteins coded by the selected genes showed that some of them are orthologs of genes reported as conferring resistance to salinity in other species. There were those coding for proteins related to the transport of salt into and out of cells, transcriptional regulatory activity, and opening and closing of stomata. The annotation analysis performed on the promoter sequence revealed 22 distinct types of cis-acting elements, and 14 of them are known to be involved in abiotic stress. Conclusion This study has helped validate the process of an accurate selection of genes responsive to salt stress with a specific and predefined expression profile and their promoter sequence. Its results also can be used in moleculargenetics-assisted breeding programs. In addition, using the identified genes is a window of opportunity for strategies trying to relieve the damages arising from the salt stress in many glycophyte crops with economic importance.
650 $aAbiotic stress
650 $aSalinity
650 $aTranscriptomics
653 $aQRT-PCR
653 $aRNA-Seq
700 1 $aFERREIRA FILHO, J. A.
700 1 $aLEAO, A. P.
700 1 $aSOUSA, C. A. F. de
700 1 $aSOUZA JUNIOR, M. T.
773 $tBMC Genomics$gv. 23, n. 735, p. 1-13, 2022.
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